What is hydroxytyrosol?

Hydroxytyrosol is a naturally-occurring polyphenol ingredient found in olives and processed olive products such as olive oil. Typically, Olive leaf extract supplement can supply natural 20% hydroxytyrosol, 25%, or 40%hydroxytyrosol powder. However, the specification of natural hydroxytyrosol can only be up to 50% on the market, and the price is insanely high for customized higher purity.

The good news is that Cima Science has been manufacturing nature-identical hydroxytyrosol liquid through the biosynthetic route, and the purity is higher than 99%. If you are interested in knowing the price and related technical documents for fermented hydroxytyrosol liquid, please send an inquiry to us.

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hydroxytyrosol liquid bulk

Hydroxytyrosol introduction

Product name Hydroxytyrosol liquid 99%
Chemical name Hydroxytyrosol; Olea25; HTEssence; 4-(2-hydroxyethyl)-benzene-1,2-diol; 3-Hydroxytyrosol; 3,4­ dihydroxyphenylethanol (DOPET); Dihydroxyphenylethanol 2-(3,4-Di-hydroxyphenyl)-ethanol (DHPE); 3,4-dihydroxyphenolethanol (3,4-DHPEA)
CAS number 10597-60-1
Chemical Formula C8H10O3
Molecular Weight 154.16
Appearance/color Slightly yellow viscous liquid
specifications 99% hydroxytyrosol
Solubility Soluble in water in all proportions; Highly soluble in polar organic solvents
Stability At least three years under recommended storage conditions
Benefits Cardiovascular health
Dosage 50 mg
Package 1kg/can, 5kg/can, 20kg/drum, 25kg/drum

Fermented hydroxytyrosol liquid is transparent golden yellow sticky oil, and is chemically 99% min pure. It is regarded to be one of the most powerful antioxidants, its oxygen radical absorbance capacity(ORAC) value is ten times higher than green tea extract, and more than two times higher than CoQ10 and quercetin.

Hydroxytyrosol bioavailability

Hydroxytyrosol has shown a high bioavailability in humans. Many studies have documented a high degree of absorption, fundamental to exert their metabolic and pharmacokinetic properties. The anticivic character facilitates its intestinal absorption and makes it exert a protective activity in both the water and lipid compartments of cells.

A small-molecule rapidly absorbed into the bloodstream and tissues, hydroxytyrosol is also able to cross the blood-brain barrier, supporting systemic defense against free radical damage.

Hydroxytyrosol Safety

Chemically-synthesized hydroxytyrosol powder or liquid is safe confirmed by both FDA in the United States and EFSA in the European Union.

Hydroxytyrosol and FDA

According to the official website of The Food and Drug Administration (FDA), hydroxytyrosol is GRAS (Generally Regarded as Safe) for use as an antioxidant in beverages, fats and oils, fresh and processed fruits and vegetables, fresh and processed fruit and vegetable juices, and gravies and sauces at a level of 5 to 10 milligrams (mg) per serving.

The safety of hydroxytyrosol is supported by multiple animal and human studies that have been performed with hydroxytyrosol, olive oil, table olives, and olive extract enriched with hydroxytyrosol. Several experimental studies, including subchronic toxicity, reproduction, and developmental toxicity, in vitro and in vivo genotoxicity and human clinical safety data support the safety in use of hydroxytyrosol at the intended use levels. Additionally, the safety of hydroxytyrosol is well established in the literature based on the dietary consumption of foods such as olive oil and table olives.

Hydroxytyrosol and EFSA

According to the article titled the safety of hydroxytyrosol as a novel food pursuant to Regulation (EC) No 258/97, hydroxytyrosol is approved as a novel food in the European Union for use in fish oils, vegetable oils and spreadable fats.

European Food Safety Authority (EFSA) has permitted health claims concerning dietary consumption of hydroxytyrosol and related polyphenol compounds from olive fruit and oil and protection of blood lipids from oxidative damage. The EFSA panel determined that a minimum of 5 mg of hydroxytyrosol and its derivatives in olive oil should be taken daily to use a cardiovascular health claim and that up to 100 mg per day was safe for adults and 200 mg was safe for older adults.

Hydroxytyrosol food sources

Hydroxytyrosol is naturally present in olives and olive oils together with other polyphenols. The phenolic compounds determine the quality of olive oil.

Hydroxytyrosol can also be found in both red and white wine in considerable amounts. Generally, in olive oil, phenols are found both as simple (hydroxytyrosol and tyrosol) and complex compounds (hydroxytyrosol or tyrosol esterified to elenolic acid, in the form of oleuropein.

The olives, leaves, and olive pulp contain large amounts of hydroxytyrosol (compared to olive oil), most of which can be recovered to produce hydroxytyrosol extracts.

Hydroxytyrosol vs. Oleuropein

Hydroxytyrosol and tyrosol are degradation products of oleuropein both in the olive fruit and in the body. Hydrolysis of oleuropein occurs in the fruit during maturation and processing, and after the ingestion of oleuropein, it is broken down by lipase activity and converted to hydroxytyrosol.

Structures of oleuropein hydroxytyrosol and tyrosol

The manufacturing process of hydroxytyrosol

There are three main methods of producing hydroxytyrosol:

  1. Natural extraction from olive leaf extract

The price is high, but the purity of hydroxytyrosol is low. 20%, 40% is the main specs on the supplement market. The natural hydroxytyrosol is low in purity, and generally in powder form. The purity of hydroxytyrosol powder can be up to 50% from Cima Science.

hydroxytyrosol bulk powder 40%

  1. Chemical synthesis method

The synthetic processes include hydrolysis, esterification, reduction, and purification steps to produce bulk hydroxytyrosol liquid 99%. The synthesis of hydroxytyrosol is based on getting an accessible source of an already naturally occurring precursor of dihydroxy phenylacetic acid. The precursor is esterified, and the resulting ester is purified and concentrated. The ester is dissolved and reduced; the excess reducing agent used is removed. The crude hydroxytyrosol is extracted. The hydroxytyrosol, thus obtained, is chemically transformed to hydroxytyrosol acetate, which is then washed with water to remove the inorganic salts, hydrolyzed, decolorized, and further purified to obtain pure hydroxytyrosol.

  1. Fermentation method

99% hydroxytyrosol liquid is obtained by a series of actions of biosynthesis, purification, and fermentation using a non-pathogenic commensal strain of Escherichia coli.

hydroxytyrosol liquid in lab bottle

The production step involves cultivation of biocatalyst E. coli, cell harvest and washing of cells to remove any trace contamination of media components, breakage of cells by homogenization to produce a broken cell preparation (biocatalyst), conversion of tyrosol substrate to hydroxytyrosol using the biocatalyst as a processing aid, followed by centrifugation to remove the biocatalyst after the reaction is completed.

Flow diagram of the fermented hydroxytyrosol production process

Hydroxytyrosol Benefits

There are many scientific studies about health benefits for hydroxytyrosol; the most prominent is cardiovascular health benefit.

Cardiovascular Health

  • Hydroxytyrosol strongly inhibits the oxidation of LDL (“bad”) cholesterol. Oxidized LDL is believed to raise the risk of cardiovascular health concerns.
  • Animal studies show that hydroxytyrosol may help to maintain blood triglycerides and cholesterol levels already within a healthy range, while also promoting HDL (“good”) cholesterol levels.
  • Hydroxytyrosol may help inhibit blood “stickiness” while blocking inflammatory signals that irritate blood vessels. These activities may support flexible arteries and clear circulation.

Hydroxytyrosol Dosage

There is no official recommended dose for hydroxytyrosol supplement. The estimated intake of hydroxytyrosol from its natural presence in olives ranges from 20-40 mg/day.

On body weight basis, the mean and high (90th percentile) intake based on USDA data is estimated as 0.42 and 0.85 mg hydroxytyrosol/kg bw/day for an individual weighing 60 kg. Based on the highest number serving of the food consumed to which hydroxytyrosol is proposed to be added, the intended use at 5 mg/serving will result in 42.5 mg/person/day or 0.71 mg/kg bw/day. For safety assessment purposes, the maximum intake of 51.06 mg hydroxytyrosol/person/day (0.85 mg/kg bw/day) is considered appropriate.

Where to buy hydroxytyrosol bulk liquid?

Most hydroxytyrosol ingredients on the supplement market are naturally extracted from olive leaves or olive oils, with purity only up to 25% or 40%.

As a manufacturer of hydroxytyrosol 99% liquid, Cima Science manufactures fermented hydroxytyrosol liquid in bulk quantities. Higher purity, more massive amounts, and lower price than the natural source.

Hydroxytyrosol 99% liquid is packed in 1kg/can, 5kg/can, 20kg/drum or 25kg/drum. If you want to buy high-quality hydroxytyrosol ingredients and want to know more about the capability, manufacturing process, pricing, certificate of analysis (COA), and relevant technical documents, please feel free to send an inquiry to us.



 

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